Contents: Anti-human FLASH (FLICE-associated huge protein) polyclonal
Catalog Number: 14-6280
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN3. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG
|
|
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The polyclonal antibody reacts with human FLICE-associated huge protein (FLASH); the antibody was raised against a synthetic peptide (serfqqlmklfekskc) corresponding to amino acids 1253 to 1268 of human origin, which differ from those of mouse by one amino acid. A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB) (0.25-0.5 μg/ml). HeLa cell lysate can be used as positive control and an approximate 220 kDa band can be detected. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85
Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3
Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7
Related Products
Cat. 18-8816 Rabbit TrueBlot™: Horseradish Peroxidase (HRP) anti-rabbit IgG
