Contents: Anti-mouse/human/rat F1Aα (FEM-1) (c-terminus) polyclonal
Catalog Number: 14-6279
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN3. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human, mouse, and rat F1Aa (FEM-1); the antibody was raised against a peptide (DINYQDQIPRTLEE) corresponding to amino acids 609 to 622 of human F1Aα. This sequence is identical to the corresponding sequence of mouse FEM1β. Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aα and FEM1β. F1Aα/FEM1β is the homologue of
C. elegans sex determining protein FEM-1. FEM-1/F1Aα is cleaved by CED-3 and caspase. FEM-1/F1Aα associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aα induces apoptosis. F1Aα is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aα is expressed in a variety of human and mouse tissues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB) (0.5-1 μg/ml). Mouse liver tissue lysate can be used as positive control and an approximately 70 kDa band can be detected. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Chan SL, Tan KO, Zhang L, Yee KS , Ronca F, Chan MY, Yu VC. F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8.
Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene. Biochem Biophys Res Commun 2000;267(1):317-20
Chan SL, Yee KS , Tan KM, Yu VC. The Caenorhabditis elegans sex determination protein FEM-1 is a CED-3 substrate that associates with CED-4 and mediates apoptosis in mammalian cells. J Biol Chem. 2000;275(24):17925-8.
(WD0102)
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