14-6271

Contents: Anti-mouse/human/rat FLIP (C-terminus) polyclonal
Catalog Number: 14-6271
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% NaN₃. Store at 4°C, stable for 6 months. Stable at -20°C for one year. Avoid multiple freeze/thaw cycles.
Clone: Polyclonal
Isotype: Rabbit IgG

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Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The polyclonal antibody reacts with human FLIP; the antibody was raised against a peptide corresponding to the C-terminus amino acids 447 to 464 of human FLIPα/FLIP_L form. There are four splice variants of Cellular FLIP (c-FLIP), FLICE-like inhibitory proteins, FLIPα, β, γ, and δ. FLIP is capable of protecting cells from death receptor-mediated apoptosis. FLIP proteins structurally resemble caspase-8 and contain two death effector domains. FLIPs are recruited to the death-inducing signaling complex and interact with adapter protein FADD, caspase-10 and caspase-8. FLIP is also known as I-FLICE, FLAME-1, CASH, CLARP and Casper.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody has been used for immunoblotting (1:500-1:2000 dilution) of FLIP from HeLa and Jurkat cell lysate as a positive control. An approximately 55 kDa band can be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

References

Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521.
Irmler M, Thome M, Hahne M, et al. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195.
Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763.
Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257.
Srinivasula SM, Ahmad M, Ottilie S, et al.. FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis. J Biol Chem 1997;272:18542-18545.
Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE, Brodianskii VM, Wallach D. CASH, a novel caspase homologue with death effector domains. J Biol Chem 1997;272:19641-19644.
Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis. Proc Natl Acad Sci USA 1997;94:10717-10722.
Wallach D. Apoptosis. Placing death under control. Nature 1997;388:123.

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