Contents: Polyclonal anti-human RAIDD
Catalog Number: 14-6258
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% sodium azide. Store at 4°C, stable for 6 months. Stable at –20°C for one year. Avoid repeated freeze/thaw cycles.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The antibody reacts with human RAIDD; the antibody was raised against residues 181-199 (C-terminus) of human RAIDD (RIP-Associated ICH-1/CED3 homologous protein with Death Domain) and recognizes the 22 kDa human RAIDD. Also known as CRADD (Caspase and RIP Adapter with Death Domain), RAIDD contains a DD and a CARD (Caspase Recruitment Domain), which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR1.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody can be used for immunoblotting (1:500-1:1000 dilution) of RAIDD from HeLa cell lysate as positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Droin, N., M. Beauchemin, et al. 2000. Identification of a caspase-2 isoform that behaves as an endogenous inhibitor of the caspase cascade. Cancer Res 60: 7039-47.
Smith, J., I. R. Paton, et al. 2000. Mapping the RAIDD gene of chicken (Gallus gallus): identification of a region homologous to the mouse high-growth region. Mamm Genome 11: 706-9.
Horvat, S. and J. F. Medrano 1998. A 500-kb YAC and BAC contig encompassing the high-growth deletion in mouse chromosome 10 and identification of the murine Raidd/Cradd gene in the candidate region. Genomics 54: 159-64.
Cohen, G. M. 1997. Caspases: the executioners of apoptosis. Biochem J 326: 1-16.
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