Product Information

Contents: Affinity Purified anti-human DcR2 (CD264, TRAIL-R4, TRAILR4, TRUNDD, TNFRSF10D)
Catalog Number: 14-6239
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: DJR4-1
Isotype: Mouse IgG1, κ

Surface staining of human DcR2 transfected cells with anti-human DcR2 (DJR4-1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-6239	PE anti-human DcR2 (CD264, TRAIL-R4, TRAILR4, TRUNDD, TNFSF10D)	488	575	FC
13-6239	Biotin anti-human DcR2 (CD264, TRAIL-R4, TRAILR4, TRUNDD, TNFSF10D)	N/A	N/A	FC
14-6239	Affinity Purified anti-human DcR2 (CD264, TRAIL-R4, TRAILR4, TRUNDD, TNFSF10D)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DJR4-1 monoclonal antibody reacts with human Decoy receptor 2 (DcR2), a transmembrane cell surface protein also known as TRAIL-R4 and TRUNDD. DcR2 has an extracellular TRAIL binding domain and a truncation in the death domain (DD) of its cytoplasmic tail. Along with DcR1, DcR2 binds to TRAIL and inhibits binding of TRAIL to DR4 or DR5 protecting cells from TRAIL-mediated cytotoxicity. DcR2 is expressed in many normal human tissues at low levels, but not in most tumors. Overexpression of DcR2 attenuates TRAIL-induced apoptosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DJR4-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DJR4-1 antibody has been tested by flow cytometric analysis of DcR2/293 transient transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control