Product Information

Contents: Affinity Purified anti-human DcR1 (CD263, TRAIL-R3, TRAILR3, LIT, TRID)
Catalog Number: 14-6238
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: DJR3
Isotype: Mouse IgG1, κ

Surface staining of human DcR1 transfected cells with anti-human DcR1 (DJR3) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6238	Affinity Purified anti-human DcR1 (CD263, TRAIL-R3, TRAILR3, LIT, TRID)	N/A	N/A	FC
13-6238	Biotin anti-human DcR1 (CD263, TRAIL-R3, TRAILR3, LIT, TRID)	N/A	N/A	FC
12-6238	PE anti-human DcR1 (CD263, TRAIL-R3, TRAILR3, LIT, TRID)	488	575	FC

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Description

The DJR3 monoclonal antibody reacts with human Decoy receptor 1 (DcR1), a glycophospholipid-anchored cell surface protein also known as TRAIL-R3, TRID, and LIT. DcR1 has an extracellular TRAIL binding domain and lacks the cytoplasmic death domain (DD) shared by several members of the TNF receptor superfamily. DcR1, along with DcR2, protects cells from TRAIL-mediated cytotoxicity. DcR1 binds to TRAIL and inhibits binding of TRAIL to DR4 or DR5. DcR1 is expressed broadly in many normal tissues at low level, but not in most tumors. Overexpression of DcR1 attenuates TRAIL-induced apoptosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DJR3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DJR3 antibody has been tested by flow cytometric analysis of human peripheral leukocytes and human DcR1-transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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