Product Information

Contents: Polyclonal anti-mouse Survivin (C-terminus)
Catalog Number: 14-6236
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% NaN₃. Store at 4°C, stable for 6 months. Stable at –20°C for one year. Avoid repeated freeze/thaw cycles.
Clone: Polyclonal

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This rabbit polyclonal antibody was raised against residues 128-140 (C-terminus) of mouse Survivin, a 17 kDa protein expressed predominantly in embryonic tissues, and in most transformed cell lines. Survivin is a member of the IAPs (Inhibitor of Apoptosis Proteins), a family of conserved proteins that bind and inhibit proteases involved in the apoptotic pathway. Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody can be used for immunoblotting (1:500-1:1000 dilution) of mouse Survivin from spleen cell lysate as a positive control. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

References

Verhagen, A. M., E. J. Coulson, et al. 2001. Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs. Genome Biol 2: REVIEWS3009.
Suzuki, A. and K. Shiraki 2001. Tumor cell "dead or alive": caspase and survivin regulate cell death, cell cycle and cell survival. Histol Histopathol 16: 583-93.
Altieri, D. C., P. C. Marchisio, et al. 1999. Survivin apoptosis: an interloper between cell death and cell proliferation in cancer. Lab Invest 79: 1327-33.
LaCasse, E. C., S. Baird, et al. 1998. The inhibitors of apoptosis (IAPs) and their emerging role in cancer. Oncogene 17: 3247-59.