Contents: Polyclonal anti-mouse Survivin (C-terminus)
Catalog Number: 14-6236
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% NaN3. Store at 4°C, stable for 6 months. Stable at –20°C for one year. Avoid repeated freeze/thaw cycles.
Clone: Polyclonal
|
|
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
This rabbit polyclonal antibody was raised against residues 128-140 (C-terminus) of mouse Survivin, a 17 kDa protein expressed predominantly in embryonic tissues, and in most transformed cell lines. Survivin is a member of the IAPs (Inhibitor of Apoptosis Proteins), a family of conserved proteins that bind and inhibit proteases involved in the apoptotic pathway. Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody can be used for immunoblotting (1:500-1:1000 dilution) of mouse Survivin from spleen cell lysate as a positive control. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
References
Verhagen, A. M., E. J. Coulson, et al. 2001. Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs. Genome Biol 2: REVIEWS3009.
Suzuki, A. and K. Shiraki 2001. Tumor cell "dead or alive": caspase and survivin regulate cell death, cell cycle and cell survival. Histol Histopathol 16: 583-93.
Altieri, D. C., P. C. Marchisio, et al. 1999. Survivin apoptosis: an interloper between cell death and cell proliferation in cancer. Lab Invest 79: 1327-33.
LaCasse, E. C., S. Baird, et al. 1998. The inhibitors of apoptosis (IAPs) and their emerging role in cancer. Oncogene 17: 3247-59.
