Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
The polyclonal antibody reacts with human and mouse MyD88; the antibody was raised against residues 279-296 of human MyD88 and recognizes human and mouse antigens. MyD88, a myeloid differentiation primary response gene, is expressed in a variety of tissues and functions as an adapter molecule in the IL-1 signaling pathway involved in the inflammatory responses induced by cytokines and LPS. MyD88 associates with and recruits IRAK to the IL-1 receptor. Dominant negative mutants of MyD88 attenuate IL-1R-mediated NF-κB activation. MyD88 also functions as a regulator molecule for IL-18 receptor and human Toll receptor family. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to LPS.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been reported for use in immunoblotting (1:500-1:1000 dilution) of MyD88 from Jurkat cell lysate as a positive control. A 35 kDa band can be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
References
Wang, Q., R. Dziarski, et al. 2001. Micrococci and peptidoglycan activate TLR2>MyD88>IRAK>TRAF>NIK>IKK>NF-kappaB signal transduction pathway that induces transcription of interleukin-8. Infect Immun 69: 2270-6. Yang, R. B., M. R. Mark, et al. 1999. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 163: 639-43. Adachi, O., T. Kawai, et al. 1998. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 9: 143-50. Hardiman, G., F. L. Rock, et al. 1996. Molecular characterization and modular analysis of human MyD88. Oncogene 13: 2467-75. Hultmark, D. 1994. Macrophage differentiation marker MyD88 is a member of the Toll/IL-1 receptor family. Biochem Biophys Res Commun 199: 144-6. Lord, K. A., B. Hoffman-Liebermann, et al. 1990. Nucleotide sequence and expression of a cDNA encoding MyD88, a novel myeloid differentiation primary response gene induced by IL6. Oncogene 5: 1095-7. Akira, S., K. Takeda, et al. 2001. Toll-like receptors: critical proteins linking innate and acquired immunity. Nat Immunol 2: 675-80.
