Product Information

Contents: Polyclonal anti-human IRAK2 (C-terminus)
Catalog Number: 14-6221
Sizes: 10 ug, 50 ug
Formulation: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% NaN₃.
Storage Conditions: Store at 4°C, stable for 6 months. Stable at –20°C for one year. Avoid multiple freeze/thaw cycles.
Clone: Polyclonal
Isotype: Rabbit IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6221	Anti-human IRAK2 polyclonal	N/A	N/A	WB

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Description

The polyclonal antibody reacts with human IRAK2; the antibody was raised against residues 571-590 of human IRAK2. IRAK2 is a member of the IRAK/Pelle family (IL-1 Receptor-associated Kinase). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members of the IRAK/Pelle family play a central role in IL-R1 and TLR mediated inflammatory responses to cytokine IL-w and LPS. IRAK2 is expressed in a variety of human tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB) and does not cross-react with IRAK.

Applications Tested

This polyclonal antibody has been tested by immunoblotting (1:500 to 1:1000 dilution) of IRAK2 from human K564 whole cells as a positive lysate. An approximately 65 kDa band can be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

References

Cao Z, Henzel WJ, Gao X. IRAK: a kinase associated with the interleukin receptor. Science1997;271:1128-31.
Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
Zhang FX, Kirschning CJ, Mancinelli, R, Xu XP, Jin Y, Faure E, Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem 1999;274:7611-4.
Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 1999;163:639-43.

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