Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The polyclonal antibody reacts with human, mouse, and rat ADAM10; the antibody was raised against residues 732-748 of human ADAM10, also known as KUZ and MADM. ADAM10 is a member of the ADAM (A Disintegrin And Metalloprotease) family of membrane-anchored glycoproteins comprised of several distinct protein modules, including a pro- and metalloprotease domain, disintegrin domain, cysteine-rich region and an EGF repeat. These proteases perform essential functions in cell adhesion and fusion in diverse systems and participate in ectodomain shedding of molecules such as TNF-α and Drosophila signaling molecule Notch.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody can be used for immunoblotting (1:500-1:2000 dilution) of ADAM10 from Jurkat cell lysate as the positive control. A 60 kDa band and an 85 kDa band can be detected corresponding to the mature and the precursor proteins, respectively. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
References
Fahrenholz, F., S. Gilbert, et al. 2000. Alpha-secretase activity of the disintegrin metalloprotease ADAM 10. Influences of domain structure. Ann N Y Acad Sci 920: 215-22. Mullberg, J., K. Althoff, et al. 2000. The importance of shedding of membrane proteins for cytokine biology. Eur Cytokine Netw 11: 27-38. Stone, A. L., M. Kroeger, et al. 1999. Structure-function analysis of the ADAM family of disintegrin-like and metalloproteinase-containing proteins. J Protein Chem 18: 447-65. Yamamoto, S., Y. Higuchi, et al. 1999. ADAM family proteins in the immune system. Immunol Today 20: 278-84. Huovila, A. P., E. A. Almeida, et al. 1996. ADAMs and cell fusion. Curr Opin Cell Biol 8: 692-9. Wolfsberg, T. G., P. Primakoff, et al. 1995. ADAM, a novel family of membrane proteins containing A Disintegrin And Metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions. J Cell Biol 131: 275-8.
