mouse/rat/human FLIP antibody, Affinity Purified conjugate

Contents: Polyclonal anti-FLIP
Catalog Number: 14-6205
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% NaN₃. Store at 4°C, stable for 6 months. Stable at –20°C for one year. Avoid multiple cycles of freeze/thaw.
Clone: Polyclonal
Isotype: Rabbit IgG

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14-6205-63

10 ug

14-6205-81

50 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6205	Anti-human, mouse, rat FLIP (N-terminus) polyclonal	N/A	N/A	WB

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Description

The polyclonal antibody reacts with human, mouse, and rat FLIP (N-terminus); the antibody was raised against amino acid 2-18 of human FLIP and recognizes all FLIP variants of human, mouse and rat origin. Cellular FLIP, FLICE-like inhibitory proteins, exist as a long c-FLIP_L and a short c-FLIP_S splice variant, both of them capable of protecting cells from death receptor-mediated apoptosis. FLIP proteins structurally resemble caspase-8 except that they lack proteolytic activity. FLIP variants are highly expressed in tumor cells, T lymphocytes and healthy, but not injured, myocytes; this suggests a critical role of FLIP as endogenous modulators of apoptosis. FLIP are recruited to the death-inducing signaling complex and interfere with the recruitment of caspase-8.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody can be used for immunoblotting (1:500-1:1000 dilution) of FLIP12 from NIH3T3 cell lysate as a positive control. An approximately 55 kDa band can be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

References

Djerbi, M., T. Darreh-Shori, et al. 2001. Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms. Scand J Immunol 54: 180-9.
Konopleva, M., S. Zhao, et al. 1999. Apoptosis. Molecules and mechanisms. Adv Exp Med Biol 457: 217-36.
Poulaki, V., N. Mitsiades, et al. 2001. Fas-mediated apoptosis in neuroblastoma requires mitochondrial activation and is inhibited by FLICE inhibitor protein and Bcl-2. Cancer Res 61: 4864-72.
Tschopp, J., M. Irmler, et al. 1998. Inhibition of fas death signals by FLIPs. Curr Opin Immunol 10: 552-8.
Krueger, A., I. Schmitz, et al. 2001. Cellular FLICE-inhibitory protein splice variants inhibit different steps of caspase-8 activation at the CD95 death-inducing signaling complex. J Biol Chem 276: 20633-40.
Kirchhoff, S., W. W. Muller, et al. 2000. TCR-mediated up-regulation of c-FLIPshort correlates with resistance toward CD95-mediated apoptosis by blocking death-inducing signaling complex activity. J Immunol 165: 6293-300.

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