Contents: Polyclonal anti-human MADD
Catalog Number: 14-6203
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% sodium azide. Store at 4°C, stable for 6 months. Stable at –20°C for one year. Avoid repeated freeze/thaw cycles.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human and mouse MADD. The antibody was raised against residues 1570-1588 of human MADD (MAP kinase-Activating Death Domain protein) and recognizes the human and mouse molecules. MADD was initially identified as the type 1 tumor necrosis factor receptor (TNFR1). Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% homology with DENN (differentially expressed in neoplastic vs. normal cells), a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94% homology with a GDP/GTP exchange protein , Rab3-GEP, and it is 87% homologous with KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signalling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein. The MADD peptide used as immunogen to generate this polyclonal antibody is identical to DENN and differs only by one amino acid with the rat GDP/GTP exchange protein Rab3-GEP.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody can be used for immunoblotting (1:250-1:500 dilution) of MADD from HeLa or NIH3T3 cell lysate as positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Rao, K. M. 2001. MAP kinase activation in macrophages. J Leukoc Biol 69: 3-10
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