Immunoblot analysis of HeLa (Lane 1) and Jurkat (Lane 2) reduced cell lysate using Anti-TACE (ADAM17) Purified at 1:500 dilution and detected using Anti-Rabbit IgG-HRP
Contents: Anti-TACE (ADAM17) Purified Catalog Number: 14-6202 Concentration: 0.5 mg/mL Formulation: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% NaN3. Storage Conditions: Store at 2-8°C. Clone: Polyclonal Host/Isotype: Rabbit IgG
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14-6202-63
10 ug
14-6202-81
50 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
The polyclonal antibody reacts with human, mouse, and rat TACE; the antibody was raised against residues 807-823 of human ADAM17, also known as TACE (TNF-α Converting Enzyme), and recognizes human, mouse and rat ADAM17. ADAM17/TACE is a member of the ADAM (A Disintegrin And Metalloprotease) family of membrane-anchored glycoproteins comprised of several distinct protein modules, including a pro- and metalloprotease domain, disintegrin domain, cysteine-rich region and an EGF repeat. ADAM17 converts TNF-α from its membrane-bound precursor (26 kDa) to generate the secreted mature form (17 kDa). ADAM17 also processes other molecules such as TNF receptor, TGF-α, and CD62L (L-Selectin). ADAM17 is expressed by a variety of human and mouse tissues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody can be used for immunoblotting (1:500-1:2000 dilution) of ADAM17 from Jurkat or HeLa cell lysate as positive control. 80-130 kDa bands can be detected representing the precursor, mature and glycosylated forms of ADAM17. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
References
Vincent, B., E. Paitel, et al. 2001. The disintegrins ADAM10 and TACE contribute to the constitutive and phorbol ester-regulated normal cleavage of the cellular prion protein. J Biol Chem 276:37743-6 Hurtado, O., A. Cardenas, et al. 2001. Up-regulation of TNF-alpha convertase (TACE/ADAM17) after oxygen-glucose deprivation in rat forebrain slices. Neuropharmacology 40:1094-102 Nelson, K. K., J. Schlondorff, et al. 1999. Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta. Biochem J 343: 673-80 Mizui, Y., K. Yamazaki, et al. 1999. cDNA cloning of mouse tumor necrosis factor-alpha converting enzyme (TACE) and partial analysis of its promoter. Gene 233: 67-74
