Contents: Affinity Purified anti-mouse/human/rat Stat5β (C-terminus) polyclonal
Catalog Number: 14-6155
Storage Conditions: This product is supplied as 1 mg/ml purified rabbit IgG fraction in PBS containing 0.2% BSA and 0.09% NaN3. Store at 4°C, stable for 12 months. Avoid multiple freeze/thaw cycles.
Clone: Polyclonal
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Description
This rabbit polyclonal antibody has been generated against the C-terminus of human Stat5β, a transcription factor activated by hormones and cytokines. Two highly related, but distinct Stat5 genes (Stat5α and Stat5β) were identified in mouse. The amino acid sequences of Stat5α and Stat5β show ~96% sequence similarity, and both proteins are co-expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat5α and Stat5β mRNA has been reported for both muscle and mammary tissue. Stat5β is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB) (5-10 μg/ml). Jurkat cells can be used as a positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Stephanou A, Latchman DS. Transcriptional regulation of the heat shock protein genes by STAT family transcription factors. Gene Expr. 1999;7(4-6):311-9.
Frank DA. STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56.
Daino H, Matsumura I, Takada K, Odajima J, Tanaka H, Ueda S, Shibayama H, Ikeda H, Hibi M, Machii T, Hirano T, Kanakura Y. Induction of apoptosis by extracellular ubiquitin in human hematopoietic cells: possible involvement of STAT3 degradation by proteasome pathway in interleukin 6-dependent hematopoietic cells. Blood. 2000 Apr 15;95(8):2577-85.
