Contents: Anti-mouse/human/rat BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor) (c-terminus) polyclonal
Catalog Number: 14-6144
Storage Conditions: This product is supplied as purified rabbit IgG in PBS containing 0.02% NaN3. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human, mouse, and rat BAFF-R; the rabbit polyclonal antibody was raised against a synthetic peptide mapping at the carboxy terminus of human BAFF-R. The peptide sequence is identical between human and mouse origin. Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF, BLyS, TALL-1, THANK, and zTNF4. BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Two receptors, TACI and BCMA, for BAFF were originally identified. A third receptor was identified recently and designated BAFF-R and BR3 for BLyS receptor 3. Unlike BCMA and TACI, which bind to BAFF and April, BAFF-R/BR3 is specific for BAFF and plays a predominant role in BAFF induced B cell development and survival. BAFF and its receptors are involved in B cell associated autoimmune diseases, and activate NF-κB and c-jun N-terminal kinase.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB) (2-5 μg/ml). Spleen cell lysate can be used as a positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Moore PA, Belvedere O, Orr A, Pieri K, LaFleur DW, Feng P, Soppet D, Charters M, Gentz R, Parmelee D, Li Y, Galperina O, Giri J, Roschke V, Nardelli B, Carrell J, Sosnovtseva S, Greenfield W, Ruben SM, Olsen HS, Fikes J, Hilbert DM. 1999. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science. Jul 9;285(5425):260-3.
Schneider P, MacKay F, Steiner V, Hofmann K, Bodmer JL, Holler N, Ambrose C, Lawton P, Bixler S, Acha-Orbea H, Valmori D, Romero P, Werner-Favre C, Zubler RH, Browning JL, Tschopp J. 1999. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med. Jun 7;189(11):1747-56.
Shu HB, Hu WH, Johnson H. 1999. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. May;65(5):680-3.
Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. 1999. Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase. J Biol Chem. Jun 4;274(23):15978-81.
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