mouse/rat/human GFRa-3 antibody, Affinity Purified conjugate

Contents: Anti-mouse/human/rat GFRα-3 (GDNFRγ) (IN) polyclonal
Catalog Number: 14-6137
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN₃. Stable for one year at 4°C.
Clone: Polyclonal

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14-6137-63

10 ug

14-6137-81

50 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6137	Anti-human, mouse, rat GFRa-3 (GDNFRg) polyclonal	N/A	N/A	WB

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Description

This rabbit polyclonal antibody was raised against a peptide corresponding to amino acids from 347 to 360 of murine GFRα-3. Members of the glial cell line-derived neurotrophic factor (GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRα and Ret receptor tyrosine kinase GFRβ. Following the findings of GFRα-1 and -2, a novel receptor in the GFRα family was identified very recently from human and mouse and designated GFRα-3. GFRα-3 binds persephin, thus, persephin, GFRα-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody has been tested by immunoblotting (WB) (1:500-1:1000). Murine kidney, liver or heart tissue lysates can be used as positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Milbrandt J, de Sauvage FJ, Fahrner TJ, Baloh RH, Leitner ML, Tansey MG, Lampe PA, Heuckeroth RO, Kotzbauer PT, Simburger KS, Golden JP, Davies JA, Vejsada R, Kato AC, Hynes M, Sherman D, Nishimura M, Wang LC, Vandlen R, Moffat B, Klein RD, Poulsen K, Gray C, Garces A, Johnson EM Jr, et al. 1998. Persephin, a novel neurotrophic factor related to GDNF and neurturin. Neuron. Feb;20(2):245-53.
Nomenclature Committee. 1997. Nomenclature of GPI-linked receptors for the GDNF ligand family. GFR(alpha) Neuron. Sep;19(3):485.
Jing S, Yu Y, Fang M, Hu Z, Holst PL, Boone T, Delaney J, Schultz H, Zhou R, Fox GM. 1997. GFRalpha-2 and GFRalpha-3 are two new receptors for ligands of the GDNF family. J Biol Chem. Dec 26;272(52):33111-7.
Baloh RH, Gorodinsky A, Golden JP, Tansey MG, Keck CL, Popescu NC, Johnson EM Jr, Milbrandt J. 1998. GFRalpha3 is an orphan member of the GDNF/neurturin/persephin receptor family. Proc Natl Acad Sci U S A. May 12;95(10):5801-6.
Worby CA, Vega QC, Chao HH, Seasholtz AF, Thompson RC, Dixon JE. 1998. Identification and characterization of GFRalpha-3, a novel Co-receptor belonging to the glial cell line-derived neurotrophic receptor family. J Biol Chem. Feb 6;273(6):3502-8.
Naveilhan P, Baudet C, Mikaels A, Shen L, Westphal H, Ernfors P. 1998. Expression and regulation of GFRalpha3, a glial cell line-derived neurotrophic factor family receptor. Proc Natl Acad Sci U S A. Feb 3;95(3):1295-300.
Nomoto S, Ito S, Yang LX, Kiuchi K. 1998. Molecular cloning and expression analysis of GFR alpha-3, a novel cDNA related to GDNFR alpha and NTNR alpha. Biochem Biophys Res Commun. Mar 27;244(3):849-53.