Contents: Anti-human NIK (NF-κB inducing kinase) (c-terminus) polyclonal
Catalog Number: 14-6128
Storage Conditions: This product is supplied as purified rabbit IgG in PBS containing 0.02% NaN3. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human NIK (NK-kB inducing kinase); the antibody was raised against a peptide corresponding to amino acids 931 to 947 of human NIK. Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF-α, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNF-α and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB). 293 cell lysate can be used as positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Malinin NL, Boldin MP, Kovalenko AV, Wallach D. 1997. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1. Nature. Feb 6;385(6616):540-4.
Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. 1997. Identification and characterization of an IkappaB kinase. Cell. Jul 25;90(2):373-83.
Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DV. 1997. IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science. Oct 31;278(5339):866-9.
Ling L, Cao Z, Goeddel DV. 1998. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci U S A. Mar 31;95(7):3792-7.
Nakano H, Shindo M, Sakon S, Nishinaka S, Mihara M, Yagita H, Okumura K. 1998. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci U S A. Mar 31;95(7):3537-42.
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