Product Information

Contents: Anti-mouse/human/rat SIRPα (SHPS-1) (c-terminus) polyclonal
Catalog Number: 14-6124
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN₃. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The polyclonal antibody reacts with human, mouse, and rat SIRPa; the antibody was raised against a peptide corresponding to amino acids 487 to 503 of human SIRPα1. It recognizes SIRPα1, 2 and 3.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody has been tested by immunoblotting (WB) (1:1000-1:2000). Whole cell lysate from THP-1 cells can be used as positive control and 75-110 kDa bands should be detected. It recognizes SIRPα1, 2 and 3. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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