14-6112

Contents: Anti-human CCR5 (CD195) (n-terminus) polyclonal
Catalog Number: 14-6112
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN₃. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG

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Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The rabbit polyclonal antibody was raised against a peptide corresponding to amino acids 6 to 20 of human CCR5. Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. Theβ-chemokines RANTES, MIP-1α and MIP-1beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody has been tested by immunoblotting (WB) (1:1000-1:2000). THP-1 whole cell lysate can be used as positive control and an approximate 40 kDa band can be detected. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Dimitrov, DS. Cell 1997;91:721-30
Littman, DR. Cell 1998;93:677-80
Deng, H, et al. Nature 1996;381:661-6
Dragic, T, et al. Nature 1996;381:667-73
Coochi, F. et al. Science 1995;270:1811-5
Wu, L. et al. Nature 1996;384:179-83
Trkola , A. et al. Nature 1996;384:184-7
Doranz, BJ. et al. J Virol 1997;71:6305-14
Dragic, T, et al. J Virol 1998;72:279-85
Raport, CJ, et al. J Biol Chem 1996;271:17161-6
Combadiere, C. et al. J Leuk Biol 1996;60:147-52

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