Contents: Anti-mouse/human/rat GPR15 (BOB)
Catalog Number: 14-6101
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN3. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human, mouse, and rat GPR15; the antibody was raised against a peptide corresponding to amino acids 13 to 28 of human GPR15. The sequence differs from those of African green monkey and pig-tailed macaque BOB by one amino acid. Human immunodeficiency virus (HIV) and related viruses require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively,) were recently cloned. This novel G protein-coupled receptor serves as corecptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 has not been identified yet.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB) (1:500-1:2000). Jurkat or A549 cell lysate can be used as a positive control and an approximately 50 kDa band can be detected. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Heiber M, Marchese A, Nguyen T, Heng HH, George SR, O'Dowd BF. 1996. A novel human gene encoding a G-protein-coupled receptor (GPR15) is located on chromosome 3. Genomics. Mar 15;32(3):462-5.
Deng HK, Unutmaz D, KewalRamani VN, Littman DR. 1997. Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature. Jul 17;388(6639):296-300.
Farzan M, Choe H, Martin K, Marcon L, Hofmann W, Karlsson G, Sun Y, Barrett P, Marchand N, Sullivan N, Gerard N, Gerard C, Sodroski J. 1997. Two orphan seven-transmembrane segment receptors which are expressed in CD4-positive cells support simian immunodeficiency virus infection. J Exp Med. Aug 4;186(3):405-11.
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