Product Information

Contents: Affinity Purified Anti-Ubiquitin Antibody
Catalog Number: 14-6078
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioP4D1 (P4D1)
Isotype: Mouse IgG1

293T cells were treated with vehicle (1) or proteosome inhibitor (2) for 4 hours. Lysates were then immunoblotted with eBioP4D1 anti-ubiquitin monoclonal antibody at 2ug/ml and revealed with anti-mouse HRP.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6078	Affinity Purified Anti-Ubiquitin Antibody	N/A	N/A	IHC IP WB
13-6078	Biotin Anti-Ubiquitin Antibody	N/A	N/A	WB

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Description

The monoclonal antibody eBioP4D1 (p4D1) recognizes ubiquitin, a small 8Ka protein. Ubiquitin units are linked to proteins via a ubiquitination process, thereby signaling the protein for degradation by the 26S proteosome. The process of ubiquitination plays a role in differentiation, cell cycle progression, apoptosis and disease progression. For example, neuronal inclusions called Lewis bodies from Parkinson patients are rich in ubiquitin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioP4D1 (P4D1) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

This eBioP4D1 (P4D1) antibody has been tested by immunblotting of treated cell lines. This can be used at less than or equal to 2 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brady M, Vlatkovic N, Boyd MT. Regulation of p53 and MDM2 activity by MTBP. Mol Cell Biol. 2005 Jan;25(2):545-53. (P4D1, IP)

Ito T, Niwa J, Hishikawa N, Ishigaki S, Doyu M, Sobue G.Dorfin localizes to Lewy bodies and ubiquitylates synphilin-1. J Biol Chem. 2003 Aug 1;278(31):29106-14. (P4D1, IHCp)

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