Contents: Anti-mouse/human/rat EndoG (endonuclease G) polyclonal
Catalog Number: 14-6035
Storage Conditions: This product is supplied as purified rabbit IgG, in PBS containing 0.02% NaN3. Store at 4°C, stable for one year.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human, mouse, and rat endoG; the antibody was raised against a synthetic peptide (ggprgpgelakyglp) corresponding to amino acids 56 to 70 of human EndoG. The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in
C. elegans. EndoG also cleaves DNA
in vitro.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB) (1-2 μg/ml). HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Li LY, Luo X, Wang X. 2001. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature. Jul 5;412(6842):95-9.
Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. 2001. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature. Jul 5;412(6842):90-4.
Hengartner MO. 2001. Apoptosis. DNA destroyers. Nature. Jul 5;412(6842):27, 29.
Widlak P, Li LY, Wang X, Garrard WT. 2001. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J Biol Chem. Dec 21;276(51):48404-9. Epub 2001 Oct 17.
Tiranti V, Rossi E, Ruiz-Carrillo A, Rossi G, Rocchi M, DiDonato S, Zuffardi O, Zeviani M. 1995. Chromosomal localization of mitochondrial transcription factor A (TCF6), single-stranded DNA-binding protein (SSBP), and endonuclease G (ENDOG), three human housekeeping genes involved in mitochondrial biogenesis. Genomics. Jan 20;25(2):559-64.
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