Contents: Anti-mouse/human/rat Stat6 (C-terminus) polyclonal
Catalog Number: 14-6016
Storage Conditions: This product is supplied as 1 mg/ml purified rabbit IgG fraction in PBS containing 0.2% BSA and 0.09% NaN3. Store at 4°C, stable for 12 months. Avoid multiple freeze/thaw cycles.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human, mouse, and rat Stat6; the antibody has been generated against the C-terminus of human Stat6, a transcription factor activated by cytokines, particularly IL-4 and IL-13. (Stat6-/-) were found to be deficient in IL-4- mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, Tcell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Stat6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. Stat6 is critically involved in Th2 immune response. The molecular weight of Stat6 is 100 kDa. Cross-reactivity with other species is not known.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of paraffin embedded tissue sections.
Applications Tested
This polyclonal antibody has been tested by immunoblotting (WB). Jurkat cells, tonsil cells or placenta cells can be used as positive controls. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Oki S, Otsuki N, Kohsaka T, Azuma M. Stat6 activation and Th2 cell differentiation [correction of proliferation] driven by CD28 [correction of CD28 signals] Eur J Immunol. 2000 May;30(5):1416-24. Erratum in: Eur J Immunol 2000 Jul;30(7):2113.
Moffatt SD, Cockman M, Metcalfe SMSTAT 6 up-regulation by FK506 in the presence of interleukin-4. Transplantation. 2000 Apr 15;69(7):1521-3.
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