Product Information

Contents: Affinity Purified anti-mouse/human Stat4
Catalog Number: 14-6014
Sizes: 10 ug, 50 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: ST4.01 (NB.34)
Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6014	Affinity purified anti-human/ mouse Stat4	N/A	N/A	IP WB

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The ST4.01 (NB.34) antibody reacts with human and mouse Stat4; the antibody was generated against the C-terminus of human Stat4. The Stat4 protein is a transcription factor activated by hormone and cytokines. Stat4 is most similar to Stat1 (52%) and Stat3 (47%). Functionally, Stat4 is similar to other Stat family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. Stat4 forms homodimers and heterodimers with related Stat family members. Tyrosine phosphorylated Stat4 can bind the IFN-γ activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. Stat4 expression is restricted to the thymus, spleen, and testis. Stat4 has an expected molecular weight of 80 kDa.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ST4.01 (NB.34) antibody has been reported for use in immunoprecipitation (mouse only), and immunoblotting (WB).

Applications Tested

The ST4.01 (NB.34) antibody has been tested by immunoblotting (WB) (1 μg/ml). Testis lysate can be used as a positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Koglin J, Glysing-Jensen T, Gadiraju S, Russell ME. Attenuated cardiac allograft vasculopathy in mice with targeted deletion of the transcription factor STAT4. Circulation. 2000 Mar 7;101(9):1034-9.
Williams JG. Serpentine receptors and STAT activation: more than one way to twin a STAT. Trends Biochem Sci. 1999 Sep;24(9):333-4.
Frank DA. STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56.

Related Products

Cat. 16-4444 Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)