Contents: Anti-human Stat1 (C-terminus) polyclonal
Catalog Number: 14-6011
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.2% BSA and 0.09% NaN3. Store at 4°C, stable for 12 months. Avoid multiple freeze/thaw cycles.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with human Stat1; the antibody was raised against the C-terminus of human Stat1, a transcription factor activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family. Stat1α (91 kDa) and Stat1β (84 kDa) isoforms arise by alternative splicing of a single gene. The only difference between these two isoforms is the absence of 38 amino acids on the C-terminus of Stat1β. Contained within these 38 C-terminal amino acids of Stat1α is a critical serine residue (Ser-727), whose phosphorylation is required for maximal transcription activity induced by IFN-γ. Cross-reactivity with other species is unknown.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (5 μg/ml). Jurkat, A431 and NIH3T3 cells can be used as positive controls. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Aittomaki S, Pesu M, Groner B, Janne OA, Palvimo JJ, Silvennoinen O. (2000) Cooperation among Stat1, glucocorticoid receptor, and PU.1 in transcriptional activation of the high-affinity Fc gamma receptor I in monocytes. J Immunol. Jun 1;164(11):5689-97.
Wilbanks AM, Mahajan S, Frank DA, Druker BJ, Gilliland DG, Carroll M. (2000) TEL/PDGFbetaR fusion protein activates STAT1 and STAT5: a common mechanism for transformation by tyrosine kinase fusion proteins. Exp Hematol. May;28(5):584-93.
Frank DA. (1999) STAT signaling in the pathogenesis and treatment of cancer. Mol Med. Jul;5(7):432-56.
Stephanou A, Latchman DS. Transcriptional regulation of the heat shock protein genes by STAT family transcription factors. Gene Expr. 1999;7(4-6):311-9.
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