Product Information

Contents: Affinity Purified anti-mouse MHC class I, H-2L^d/H-2D^b
Catalog Number: 14-5999
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 28-14-8
Isotype: Mouse IgG2a, κ

Surface staining of mouse splenocytes with anti-mouse MHC Class I (28-14-8) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5999	Affinity Purified anti-mouse MHC Class I	N/A	N/A	FC
16-5999	Functional Grade* Purified anti-mouse MHC Class I	N/A	N/A	FC
13-5999	Biotin anti-mouse MHC Class I	N/A	N/A	FC
11-5999	FITC anti-mouse MHC Class I	488	518	FC
12-5999	PE anti-mouse MHC Class I	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on β₂-microglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 28-14-8 has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, immunoprecipitation and complement-dependent cytotoxicity.

Applications Tested

The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 1 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ozato, K., T. H. Hansen, et al. (1980). "Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex." J Immunol 125(6): 2473-7.
Ozato, K., N. Mayer, et al. (1980). "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." J Immunol 124(2): 533-40.

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