Product Information

Contents: Affinity Purified anti-mouse MAdCAM-1
Catalog Number: 14-5997
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MECA-367
Isotype: Rat IgG2a, κ

Surface staining of mouse TNF-a stimulated bEnd.3 cells with anti-mouse MAdCAM-1 (MECA-367) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5997	Affinity Purified anti-mouse MAdCAM-1	N/A	N/A	FC
16-5997	Functional Grade* Purified anti-mouse MAdCAM-1	N/A	N/A	FC
13-5997	Biotin anti-mouse MAdCAM-1	N/A	N/A	FC
11-5997	FITC anti-mouse MAdCAM-1	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MECA-367 monoclonal antibody reacts with mouse mucosal vascular addressin-1 (MAdCAM-1), a 50-60 kDa member of the Ig superfamily. This antigen is expressed in mucosal lymphoid tissue and lamina propria and is a counter-receptor for CD62L and CD49d. MAdCAM-1 is involved in homing of subpopulation of memory T cells to the gut lamina propria, and recirculation of naïve lymphocytes in Peyer’s patches and mesenteric lymph nodes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MECA-367 can be used in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, blocking in adhesion assays, immunoprecipitation and immunoblotting (WB).

Applications Tested

The MECA-367 antibody has been tested by immunohistochemical staining of frozen tissues and can be used at 10 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Streeter, P. R., B. T. Rouse, et al. (1988). "Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes." J Cell Biol 107(5): 1853-62.
Streeter, P. R., E. L. Berg, et al. (1988). "A tissue-specific endothelial cell molecule involved in lymphocyte homing." Nature 331(6151): 41-6.

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