Product Information

Contents: Affinity Purified anti-mouse Qa-2 (Qa2)
Catalog Number: 14-5996
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 69H1-9-9
Isotype: Mouse IgG2a, κ

Surface staining of mouse splenocytes with anti-mouse Qa-2 (69H1-9-9) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5996	Affinity Purified anti-mouse Qa-2 (Qa2)	N/A	N/A	FA FC
11-5996	FITC anti-mouse Qa-2 (Qa2)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 69H1-9-9 monoclonal antibody reacts with the mouse Qa-2 molecule, a GPI-linked MHC class Iβ surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level is different depending on the laboratory mouse strain.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 69H1-9-9 antibody has been reported for use in flow cytometric analysis. It has alaso been reported in complement-dependent cytotoxicity.

Applications Tested

The 69H1-9-9 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 1 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sharrow, S. O., J. S. Arn, et al. (1989). "Epitope clusters of Qa-2 antigens defined by a panel of new monoclonal antibodies." J Immunol 142(10): 3495-502.

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