Product Information

Contents: Affinity Purified anti-mouse IgA
Catalog Number: 14-5994
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.1% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 11-44-2
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5994	Affinity Purified anti-mouse IgA	N/A	N/A	FC
13-5994	Biotin anti-mouse IgA	N/A	N/A	FC
12-5994	PE anti-mouse IgA	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 11-44-2 monoclonal antibody reacts with the α heavy chain of mouse IgA. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgM. IgA is expressed as a membrane-bound form as well as a soluble dimer. Fluorochrome conjugated 11-44-2 is used as a detection secondary for mouse IgA.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 11-44-2 has been reported for use in flow cytometric analysis to detect the surface IgA on mouse B cells and as a second step to detect primary mouse IgAs. It can also be used in ELISA.

Applications Tested

The fluorochrome conjugated 11-44-2 has been tested by flow cytometric analysis to detect a mouse IgA primary antibody and can be used at less than or equal to 1 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 14-4301  Affinity Purified Rat IgG1 Isotype Control
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)