Product Information

Contents: Affinity Purified anti-mouse Mac-3
Catalog Number: 14-5989
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: M3/84
Isotype: Rat IgG1, κ

Surface staining of thioglycolate induced PEC with anti-mouse Mac-3 (M3/84) FITC (left), and PE (right). Autofluorescence is shown via open histogram. Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5989	Affinity Purified anti-mouse Mac-3	N/A	N/A	FC IH/F IP
11-5989	FITC anti-mouse Mac-3	488	518	FC
12-5989	PE anti-mouse Mac-3	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The M3/84 monoclonal antibody reacts with mouse Mac-3, the 110 kDa protein expressed by mononuclear phagocytes. The Mac-3 antigen is expressed intracellularly by bone marrow monocyte lineage and is upregulated during macrophage differentiation. Peritoneal and tissue macrophages, dendritic cells, and endothelial cells express this antigen on their surface. It is reported that Mac-3 may be identical to CD107b.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The M3/84 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunoprecipitation.

Applications Tested

The M3/84 antibody has been tested by flow cytometric analysis of mouse bone marrow and thioglycolate-elicited peritoneal exudates cell suspensions and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Flotte, T. J., T. A. Springer, et al. (1983). "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." Am J Pathol 111(1): 112-24.
Ho, M. K. and T. A. Springer (1983). "Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity." J Biol Chem 258(1): 636-42.
Springer, T., G. Galfre, et al. (1978). "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." Eur J Immunol 8(8): 539-51.

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