PLEASE NOTE: The MIH6 clone is being discontinued. We recommend clone MIH5, a better characterized sister clone, as an alternate product:
click here for more information
Contents: Affinity Purified anti-mouse B7-H1 (PD-L1)
Catalog Number: 14-5984
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH6
Isotype: Rat IgG2a
|
|
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MIH6 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. Preliminary experiments suggest that MIH6 may not be a blocking antibody. For blocking in functional assays, clone MIH5 (catalog # 16-5982 ) is recommended.
Applications Reported
For research use only, not for diagnostic or therapeutic use. MIH6 has been reported for use in flow cytometric analysis, immunoprecipitation and immunoblotting (non-reducing and reducing conditions).
References
Dong, H., G. Zhu, et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med 5(12): 1365-9.
Azuma, M. 2002. personal communication.
Freeman, G. J., A. J. Long, et al. 2000. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.
