human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) antibody: purified antibody

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Contents: Affinity Purified anti-human B7-H1 (CD274, B7H1, PDL-1, PD-L1)
Catalog Number: 14-5983
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH1
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-5983-80	25 ug	$85
14-5983-82	100 ug	$185

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.5 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.5 μg of Purified anti-human B7-H1 (MIH1) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5983	Affinity Purified anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FC IH/F WB
16-5983	Functional Grade* Purified anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FA FC
13-5983	Biotin anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FC
12-5983	PE anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	488	575	FC
17-5983	Allophycocyanin (APC) anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	633	660	FC
25-5983	Phycoerythrin-Cy7 (PE-Cy7) anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking in in vitro functional assays. (Please use Functional Grade purified MIH1, cat. 16-5983, in functional assays.)

Applications Tested

The MIH1 antibody has been tested by flow cytometric analysis of human peripheral leukocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Dong, H., G. Zhu, et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med 5(12): 1365-9.

Freeman, G. J., A. J. Long, et al. 2000. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. 2003. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 171(8):4156-63.

Saudemont, A., Jouy, N., Hetuin, D., and Quesnel, B. 2005. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 105:2428-2435.

Ohigashi Y, Sho M, Yamada Y, Tsurui Y, Hamada K, Ikeda N, Mizuno T, Yoriki R, Kashizuka H, Yane K, Tsushima F, Otsuki N, Yagita H, Azuma M, Nakajima Y. Clinical significance of programmed death-1 ligand-1 and programmed death-1 ligand-2 expression in human esophageal cancer. Clin Cancer Res. 2005 Apr 15;11(8):2947-53. (MIH1, IH/F, PubMed)

Chen Y, Zhang J, Li J, Zou L, Zhao T, Tang Y, Wu Y. Expression of B7-H1 in inflammatory renal tubular epithelial cells. Nephron Exp Nephrol. 2006;102(3-4):e81-92. (MIH1, IH/F, FA, PubMed)

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Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
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Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control