Anti-Human CD274 (B7-H1) Purified

Also known as: B7H1, PD-L1, PDL-1, PDL1

RUO: For Research Use Only

human B7-H1 antibody, Affinity Purified conjugate

Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 κ Isotype Control Purified (cat. 14-4714) (open histogram) or 0.5 µg of Anti-Human CD274 (B7-H1) Purified (filled histogram) followed by Anti-Mouse IgG Biotin (cat. 13-4013)and Streptavidin PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Contents: Anti-Human CD274 (B7-H1) Purified
Catalog Number: 14-5983
Concentration: 0.5 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 2-8°C.
Clone: MIH1
Host/Isotype: Mouse IgG1

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-5983-80	25 ug	To view relevant pricing, please select your country and resume browsing.
14-5983-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4011	Anti-Mouse IgG FITC
11-4317	Streptavidin FITC
12-4317	Streptavidin PE
13-4013	Anti-Mouse IgG Biotin (Polyclonal)
14-4714	Mouse IgG1 K Isotype Control Purified
17-4317	Streptavidin APC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-5983	Anti-Human CD274 (B7-H1) PE	488	575	FC	RUO
13-5983	Anti-Human CD274 (B7-H1) Biotin	N/A	N/A	FC	RUO
14-5983	Anti-Human CD274 (B7-H1) Purified	N/A	N/A	FC IHC (F) WB	RUO
16-5983	Anti-Human CD274 (B7-H1) Functional Grade* Purified	N/A	N/A	FA FC	RUO
17-5983	Anti-Human CD274 (B7-H1) APC	633	660	FC	RUO
25-5983	Anti-Human CD274 (B7-H1) PE-Cy7	488	760	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking in in vitro functional assays. (Please use Functional Grade purified MIH1, cat. 16-5983, in functional assays.)

Applications Tested

The MIH1 antibody has been tested by flow cytometric analysis of human peripheral leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Dong, H., G. Zhu, et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med 5(12): 1365-9.

Freeman, G. J., A. J. Long, et al. 2000. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. 2003. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 171(8):4156-63.

Saudemont, A., Jouy, N., Hetuin, D., and Quesnel, B. 2005. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 105:2428-2435.

Ohigashi Y, Sho M, Yamada Y, Tsurui Y, Hamada K, Ikeda N, Mizuno T, Yoriki R, Kashizuka H, Yane K, Tsushima F, Otsuki N, Yagita H, Azuma M, Nakajima Y. Clinical significance of programmed death-1 ligand-1 and programmed death-1 ligand-2 expression in human esophageal cancer. Clin Cancer Res. 2005 Apr 15;11(8):2947-53. (MIH1, IH/F, PubMed)

Chen Y, Zhang J, Li J, Zou L, Zhao T, Tang Y, Wu Y. Expression of B7-H1 in inflammatory renal tubular epithelial cells. Nephron Exp Nephrol. 2006;102(3-4):e81-92. (MIH1, IH/F, FA, PubMed)

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.