mouse B7-H1 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-mouse B7-H1 (CD274, B7H1, PDL-1, PD-L1)
Catalog Number: 14-5982
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH5
Isotype: Rat IgG2a, λ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
14-5982-81	50 ug
14-5982-82	100 ug
14-5982-85	500 ug

Surface staining of mouse splenocytes with anti-mouse B7-H1 (MIH5) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5982	Affinity Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FA FC IH/F WB
16-5982	Functional Grade* Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FA FC
13-5982	Biotin anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FC
12-5982	PE anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking in in vitro and in vivo functional assays. (Please use Functional Grade purified MIH5, cat. 16-5982, in functional assays.)

Applications Tested

The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sheng H, Wang Y, Jin Y, Zhang Q, Zhang Y, Wang L, Shen B, Yin S, Liu W, Cui L, Li N. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res. 2008 Aug;18(8):846-57. (MIH5, FC, PubMed)

Saudemont A, Jouy N, Hetuin D, Quesnel B. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 2005 Mar 15;105(6):2428-35. (MIH5, FA, PubMed)

Schoop R, Wahl P, Le Hir M, Heemann U, Wang M, Wüthrich RP. Suppressed T-cell activation by IFN-gamma-induced expression of PD-L1 on renal tubular epithelial cells. Nephrol Dial Transplant. 2004 Nov;19(11):2713-20. (MIH5, FA, WB, IHC frozen, PubMed)

Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, Azuma M, Okumura K, Yagita H, Niimi M. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. 2004 Jan 15;77(1):6-12.

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003 Oct 15;171(8):4156-63.

Tsushima F, Iwai H, Otsuki N, Abe M, Hirose S, Yamazaki T, Akiba H, Yagita H, Takahashi Y, Omura K, Okumura K, Azuma M. Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses. Eur J Immunol. 2003 Oct;33(10):2773-82. (MIH5, FA, IHC frozen)

Freeman GJ, Long AJ, Iwai Y, Bourque K, Chernova T, Nishimura H, Fitz LJ, Malenkovich N, Okazaki T, Byrne MC, Horton HF, Fouser L, Carter L, Ling V, Bowman MR, Carreno BM, Collins M, Wood CR, Honjo T. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000 Oct 2;192(7):1027-34.

Dong H, Zhu G, Tamada K, Chen L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999 Dec;5(12):1365-9.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321  Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)