Product Information

Contents: Affinity Purified anti-mouse MHC class II (I-E^k)
Catalog Number: 14-5980
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 14-4-4S
Isotype: Mouse IgG2a, κ

Staining of BALB/c splenocytes with 0.25 µg of purified mouse IgG2a isotype control (cat.14-4724) (open histogram) or 0.25 µg of purified 14-4-4S (colored histogram) followed by FITC anti-mouse IgG2a (cat.11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5980	Affinity Purified anti-mouse/rat MHC class II (I-Ek)	N/A	N/A	FC
13-5980	Biotin anti-mouse/rat MHC class II (I-Ek)	N/A	N/A	FC
11-5980	FITC anti-mouse/rat MHC class II (I-Ek)	488	518	FC
12-5980	PE anti-mouse/rat MHC class II (I-Ek)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 14-4-4S has been reported for use in flow cytometric analysis, immunoprecipitation, complement-dependent cytotoxity and immunohistochemical staining of frozen sections (Biotinylated 14-4-4S, cat.13-5980, is recommended for IHC).

Applications Tested

The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocyte suspension and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Farr A, DeRoos PC, Eastman S, Rudensky AY. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol. 1996 Dec;26(12):3185-93.
Spencer JS, Freed JH, Kubo RT. Expression and function of mixed isotype MHC class II molecules in normal mice. J Immunol. 1993 Dec 15;151(12):6822-32.
Pruitt SK, Baldwin WM 3rd, Barth RN, Sanfilippo F. The effect of xenoreactive antibody and B cell depletion on hyperacute rejection of guinea pig-to-rat cardiac xenografts. Transplantation. 1993 Dec;56(6):1318-24.
Liu H, Steiner BM, Alder JD, Baertschy DK, Schell RF. Immune T cells sorted by flow cytometry confer protection against infection with Treponema pallidum subsp. pertenue in hamsters. Infect Immun. 1990 Jun;58(6):1685-90.
Maloy WL, Ozato K, Sachs DH, Coligan JE. Evidence for the association of I-A and I-E molecules in d-haplotype mice. Mol Immunol. 1986 Mar;23(3):263-9.

Related Products

Cat. 11-4011 FITC Anti-Mouse IgG
Cat. 14-4724 Affinity Purified Mouse IgG2a, K Isotype Control