Contents: Affinity Purified anti-human BTLA (CD272, B and T lymphocyte attenuator)
Catalog Number: 14-5979
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH26
Isotype: Mouse IgG2a
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Staining of normal human peripheral blood cells with .25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or .25 μg of Purified anti-human BTLA (MIH26) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-5979 |
Affinity Purified anti-human BTLA (CD272, B and T lymphocyte attenuator) |
N/A |
N/A |
FC
|
| 16-5979 |
Functional Grade* Purified anti-human BTLA (CD272, B and T lymphocyte attenuator) |
N/A |
N/A |
FC
|
| 13-5979 |
Biotin anti-human BTLA (CD272, B and T lymphocyte attenuator) |
N/A |
N/A |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The BTLA monoclonal antibody reacts with human B and T lymphocyte attenuator (BTLA). BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in the thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This MIH26 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This MIH26 antibody has been tested by flow cytometric analysis of nomral human peripheral blood. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Watanabe N, Gavrieli M, Sedy JR, Yang J, Fallarino F, Loftin SK, Hurchla MA, Zimmerman N, Sim J, Zang X, Murphy TL, Russell JH, Allison JP, Murphy KM.
BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1.
Nat Immunol. 2003 Jul;4(7):670-9.
Han P, Goularte OD, Rufner K, Wilkinson B, Kaye J.
An inhibitory Ig superfamily protein expressed by lymphocytes and APCs is also an early marker of thymocyte positive selection.
J Immunol. 2004 May 15;172(10):5931-9.
Sedy JR, Gavrieli M, Potter KG, Hurchla MA, Lindsley RC, Hildner K, Scheu S, Pfeffer K, Ware CF, Murphy TL, Murphy KM.
B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator.
Nat Immunol. 2005 Jan;6(1):90-8.
Related Products
Cat. 14-4724 Affinity Purified Mouse IgG2a, K Isotype Control
Human Cellular Antigens Chart