Product Information

Contents: Affinity Purified anti-mouse B7-H3 (CD276, B7H3)
Catalog Number: 14-5973
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: M3.2D7
Isotype: Rat IgG2a

Staining of non-transfected (left) and mouse B7-H3 transfected (right) 293T cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified M3.2D7 (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5973	Purified anti-mouse/human CD276 (B7RP-2, B7H3)	N/A	N/A	FC
16-5973	Functional Grade* Purified anti-mouse/human CD276 (B7RP-2, B7H3)	N/A	N/A	FC
13-5973	Biotin anti-mouse/human CD276 (B7RP-2, B7H3)	N/A	N/A	FC
12-5973	Phycoerythrin (PE) anti-mouse/human CD276 (B7RP-2, B7H3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The M3.2D7 monoclonal antibody was generated against and reacts with mouse B7-H3, a member of the B7 family of the Ig superfamily proteins. B7-H3 is reported to be expressed by antigen presenting cells and developing bone during embryogenesis. The ligand for B7-H3 has not yet been identified and it is thought that B7-H3 down-regulates T cell functions by engaging an unknown counter receptor on T cells. M3.2D7 stains mouse B7-H3 transfected cells and not mouse spleen cells. M3.2D7 also stains human B7-H3 transfected cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The M3.2D7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The M3.2D7 antibody has been tested by flow cytometric analysis of mB7-H3 transfeted cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Chen Y, Yang C, et al. 2007. Terminal complement complex C5b-9-treated human monocyte-derived dendritic cells undergo maturation and induce Th1 polarization. Eur J Immunol. 37(1):167-76. (human FC, PubMed)

Chen Y, Yang C, et al. 2007. Sinomenine promotes differentiation but impedes maturation and co-stimulatory molecule expression of human monocyte-derived dendritic cells. Int Immunopharmacol. 7(8):1102-10. (human FC, PubMed)

Nakae S, Iwakura Y, et al. 2007. Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17. J Leukoc Biol. 81(5):1258-68. (mouse FC, PubMed)

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