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Affinity Purified anti-mouse pan-NK cells (CD49b, α2 integrin, very late antigen-2)
 
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Contents: Affinity Purified anti-mouse pan-NK cells (CD49b, α2 integrin, very late antigen-2)
Catalog Number: 14-5971
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: DX5
Isotype: Rat IgM, κ

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14-5971-81 50 ug
14-5971-82 100 ug
14-5971-85 500 ug


   
data image 1 data image 2
Staining of C57Bl/6 splenocytes with FITC anti-mouse NK-1.1 (PK136) (cat. 11-5941) and .25 μg of Purified Rat IgM Iso Cntrl (cat. 14-4341) (left) or .25 μg of Purified anti-mouse pan-NK (DX5) (right) followed by PE anti-rat IgM (HIS40) (cat. 12-0990). Cells in the lymphocyte gate were used for analysis.
Available Formats of This Product
Cat. No. Format Excite
(nm)		 Emit
(nm)		 Reported Applications
14-5971 Affinity Purified anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) N/A N/A FC 
13-5971 Biotin anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) N/A N/A FC 
11-5971 FITC anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) 488 518 FC 
12-5971 PE anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) 488 575 FC 
17-5971 APC anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) 633 660 FC 
25-5971 Phycoerythrin-Cy7 (PE-Cy7) anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) 488 760 FC 
36-5971 Functional Grade* Biotin anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) N/A N/A FC 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DX5 antibody has been reported for use in flow cytometric analysis. This antibody does not block adhesion and function of NK cells, and is not useful for immunoprecipitation and immunohistochemical staining of mouse tissues.

Applications Tested

The DX5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References


Hussell, T. and P. J. Openshaw (1998). "Intracellular IFN-gamma expression in natural killer cells precedes lung CD8+ T cell recruitment during respiratory syncytial virus infection." J Gen Virol 79(Pt 11): 2593-601.
Vos, Q., J. R. Ortaldo, et al. (1998). "Phenotypic and functional characterization of a panel of cytotoxic murine NK cell clones that are heterogeneous in their enhancement of Ig secretion in vitro." Int Immunol 10(8): 1093-101.
Arase, H., T. Saito, et al. (2001). Cutting edge: the mouse NK cell-associated antigen recognized by DX5 moncoclonal antibody is CD49b (alpha(2) integrin, very late antigen-2). J Immunol 167(3): 1141-4.

Related Products

Cat. 11-0990  FITC anti-rat IgM (clone HIS40)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4341  Affinity Purified Rat IgM Isotype Control
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