Product Information

Contents: Affinity Purified anti-human HVEM (herpes virus entry mediator)
Catalog Number: 14-5969
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioHVEM-122
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human HVEM (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5969	Affinity Purified anti-human HVEM (herpes virus entry mediator)	N/A	N/A	FC WB
13-5969	Biotin anti-human HVEM (herpes virus entry mediator)	N/A	N/A	FC
12-5969	Phycoerythrin (PE) anti-human HVEM (herpes virus entry mediator)	488	575	FC
17-5969	Allophycocyanin (APC) human HVEM (herpes virus entry mediator)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioHVEM-122 antibody reacts with Herpes Virus Entry Mediator (HVEM, TR2), a member of the TNF-receptor superfamily. HVEM is found on most cell types, including T cells, B cells, monocytes, neutrophils and dendritic cells. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of HVEM was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. Recent studies have shown HVEM as a unique ligand for BTLA (B and T lymphocyte attenuator). The conservation of the BTLA-HVEM interaction between mouse and human suggests that this system is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBioHVEM-122 antibody has been reported for use in flow cytometric analysis and immunoblotting.

Applications Tested

The eBioHVEM-122 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 µg per 100 µl blood (or per 1 million cells in 100 µl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Jung HW, La SJ, Kim JY, Heo SK, Kim JY, Wang S, Kim KK, Lee KM, Cho HR, Lee HW, Kwon B, Kim BS, Kwon BS. High levels of soluble herpes virus entry mediator in sera of patients with allergic and autoimmune diseases. Exp Mol Med. 2003 Dec 31;35(6):501-8. (eBioHVEM-122, FC, PubMed)

Heo SK, Ju SA, Lee SC, Park SM, Choe SY, Kwon B, Kwon BS, Kim BS. LIGHT enhances the bactericidal activity of human monocytes and neutrophils via HVEM. J Leukoc Biol. 2006 Feb;79(2):330-8. (PubMed)

Sedy JR, Gavrieli M, Potter KG, Hurchla MA, Lindsley RC, Hildner K, Scheu S, Pfeffer K, Ware CF, Murphy TL, Murphy KM. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. 2005 Jan;6(1):90-8. (PubMed)

Morel Y, Schiano de Colella JM, Harrop J, Deen KC, Holmes SD, Wattam TA, Khandekar SS, Truneh A, Sweet RW, Gastaut JA, Olive D, Costello RT. Reciprocal expression of the TNF family receptor herpes virus entry mediator and its ligand LIGHT on activated T cells: LIGHT down-regulates its own receptor. J Immunol. 2000 Oct 15;165(8):4397-404. (PubMed)

Kwon BS, Tan KB, Ni J, Oh KO, Lee ZH, Kim KK, Kim YJ, Wang S, Gentz R, Yu GL, Harrop J, Lyn SD, Silverman C, Porter TG, Truneh A, Young PR. A newly identified member of the tumor necrosis factor receptor superfamily with a wide tissue distribution and involvement in lymphocyte activation. J Biol Chem. 1997 May 30;272(22):14272-6. (PubMed)

Related Products

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