Product Information

Contents: Affinity Purified anti-mouse TCRβ
Catalog Number: 14-5961
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: H57-597
Isotype: Armenian Hamster IgG

BALB/c splenocytes were stained with purified anti-mouse TCRβ (H57-597), followed by FITC anti-Armenian Hamster IgG. Viable cells were analyzed. The red histogram shows staining with the second step alone, and the green histogram shows staining of TCRαβ-positive splenocytes with H57-597.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5961	FITC anti-mouse TCR beta (TCRb)	488	518	FC
12-5961	PE anti-mouse TCR beta (TCRb)	488	575	FC
13-5961	Biotin anti-mouse TCR beta (TCRb)	N/A	N/A	FC
14-5961	Affinity Purified anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC IHC
15-5961	PE-Cy5 anti-mouse TCR beta (TCRb)	488	670	FC
16-5961	Functional Grade* Purified anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC
17-5961	APC anti-mouse TCR beta (TCRb)	633	660	FC
27-5961	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TCR beta (TCRb) (APC-Cy7 Equivalent)	633	779	FC
35-5961	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse TCR beta (TCRb)	488	690	FC
36-5961	Functional Grade* Biotin anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC IHC
45-5961	PerCP-Cy5.5 anti-mouse TCR beta (TCRb)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The H57-597 monoclonal antibody reacts with the β chain of mouse TCR. TCRβ is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR β expressing T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The H57-597 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. It has also been reported in in vitro functional studies and in vivo depletion. (Please use Functional Grade purified H57-597, cat. 16-5961, in functional assays.)

Applications Tested

The H57-597 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kubo, R. T., W. Born, et al. (1989). "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." J Immunol 142(8): 2736-42.

Related Products

Cat. 11-4111    FITC Anti-Armenian Hamster IgG
Cat. 13-4113    Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4444    Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 14-4888    Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)