Affinity Purified anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)

Contents: Affinity Purified anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)
Catalog Number: 14-5943
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio7H22-E16
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-5943-81	50 ug	$55
14-5943-82	100 ug	$95

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes 0.5 μg of Purified Rat IgG1 Iso Cntrl (cat. 14-4301) (left) or 0.5 μg of Purified anti-mouse BAFF R (eBio7H22-E16) (right) followed by Biotin Anti-Rat IgG (cat. 13-4813) and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5943	Affinity Purified anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	N/A	N/A	FC WB
11-5943	Fluorescein isothiocyanate (FITC) anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	488	518	FC
51-5943	Alexa Fluor® 647 anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	633	668	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio7H22.E16 reacts with mouse BAFF-Receptor, a type III transmembrane TNF receptor family member. BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice. The receptor is found on the surface of all B220+ B cells found in spleen. BAFF-R is expressed on activated/memory subsets of T-cells and is important for splenic B cell maturation and survival and is a major mediator of BAFF- dependent costimulatory responses in peripheral B and T cells. BAFF-R can cause a high level of autoimmune disease and a defect in BAFF-R will cause a decrease in generating mature B-Cells The ligand for the BAFF-R is BAFF also known as BLyS.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio7H22-E16 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB) under reducing conditions.

Applications Tested

This eBio7H22-E16 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1.0 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ng LG, Ng CH, Woehl B, Sutherland AP, Huo J, Xu S, Mackay F, Lam KP. BAFF costimulation of Toll-like receptor-activated B-1 cells. Eur J Immunol. 2006 Jul;36(7):1837-46. (7H22-E16, FC Pubmed)

Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol. 2004 Jul 15;173(2):807-17.(7H22-E16, FC PubMed)

Thompson JS, Bixler SA, Qian F, Vora K, Scott ML, Cachero TG, Hession C, Schneider P, Sizing ID, Mullen C, Strauch K, Zafari M, Benjamin CD, Tschopp J, Browning JL, Ambrose C. BAFF-R, a newly identified TNF receptor that specifically interacts with BAFF. Science. 2001 Sep 14;293(5537):2108-11

Related Products

Cat. 14-4301    Affinity Purified Rat IgG1 Isotype Control
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)