Anti-Mouse Ly-6G (Gr-1) Purified

Also known as: Gr1, Ly6G

RUO: For Research Use Only

mouse Ly-6G antibody, Affinity Purified conjugate

Staining of mouse bone marrow with Anti-Mouse Ly-6G (Gr-1) FITC and Anti-Human/Mouse CD45R (B220) PE (cat. 12-0452). Total viable cells were used for analysis

Contents: Anti-Mouse Ly-6G (Gr-1) Purified
Catalog Number: 14-5931
Concentration: 0.5 mg/ml
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 2-8°C.
Clone: RB6-8C5
Host/Isotype: Rat IgG2b, κ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-5931-81	50 ug	To view relevant pricing, please select your country and resume browsing.
14-5931-82	100 ug
14-5931-85	500 ug
14-5931-86	1 mg

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4317	Streptavidin FITC
11-4811	Anti-Rat IgG FITC
12-4317	Streptavidin PE
13-4813	Anti-Rat IgG Biotin (Polyclonal)
14-4031	Rat IgG2b K Isotype Control Purified
17-4317	Streptavidin APC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-5931	Anti-Mouse Ly-6G (Gr-1) FITC	488	518	FC	RUO
12-5931	Anti-Mouse Ly-6G (Gr-1) PE	488	575	FC	RUO
13-5931	Anti-Mouse Ly-6G (Gr-1) Biotin	N/A	N/A	FC	RUO
14-5931	Anti-Mouse Ly-6G (Gr-1) Purified	N/A	N/A	FA FC IHC (F) IHC (FFPE) IP WB	RUO
15-5931	Anti-Mouse Ly-6G (Gr-1) PE-Cy5	488	670	FC	RUO
16-5931	Anti-Mouse Ly-6G (Gr-1) Functional Grade* Purified	N/A	N/A	FA FC	RUO
17-5931	Anti-Mouse Ly-6G (Gr-1) APC	633	660	FC	RUO
19-5931	Anti-Mouse Ly-6G (Gr-1) Cy5	633	670	FC	RUO
25-5931	Anti-Mouse Ly-6G (Gr-1) PE-Cy7	488	760	FC	RUO
35-5931	Anti-Mouse Ly-6G (Gr-1) PE-Cy5.5 (To Be Discontinued; See Replacement Formats: PerCP-Cy5.5)	488	690	FC	RUO
36-5931	Anti-Mouse Ly-6G (Gr-1) Functional Grade* Biotin	N/A	N/A	FC	RUO
45-5931	Anti-Mouse Ly-6G (Gr-1) PerCP-Cy5.5 (PE-Cy5.5 replacement)	488	690	FC	RUO
47-5931	Anti-Mouse Ly-6G (Gr-1) APC-eFluor® 780	633	780	FC	RUO
48-5931	Anti-Mouse Ly-6G (Gr-1) eFluor® 450 (Pacific Blue® replacement)	405	450	FC	RUO
50-5931	Anti-Mouse Ly-6G (Gr-1) eFluor® 660 (Alexa Fluor® 647 Replacement)	633	660	FC	RUO
51-5931	Anti-Mouse Ly-6G (Gr-1) Alexa Fluor® 647 (To Be Discontinued. Refer to NEW Format: eFluor® 660, cat. 50-5931)	633	668	FC	RUO
53-5931	Anti-Mouse Ly-6G (Gr-1) Alexa Fluor® 488	488	519	FC	RUO
56-5931	Anti-Mouse Ly-6G (Gr-1) Alexa Fluor® 700	633	723	FC	RUO
94-5931	Anti-Mouse Ly-6G (Gr-1) eFluor® 625NC	350-500	625	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.
Blocking studies with Ly-6C (clone HK1.4) or RB6-8C5 show no effect against staining with the other clone thereby suggesting that RB6-8C5 does not recognize Ly-6C in resting bone marrow or splenocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RB6-8C5 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining. It has also been reported in cell depletion. (Please use Functional Grade purified RB6-8C5, cat. 16-5931, in functional assays.)

Applications Tested

The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sasmono RT, Ehrnsperger A, Cronau SL, Ravasi T, Kandane R, Hickey MJ, Cook AD, Himes SR, Hamilton JA, Hume DA. Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1. J Leukoc Biol. 2007 Jul;82(1):111-23. (RB6-8C5, Cross-reactivity to Ly-6G and Ly-6C, Pubmed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (RB6-8C5, in vivo depletion, PubMed)

Fan J, Li Y, Vodovotz Y, Billiar TR, Wilson MA. Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation. Am J Physiol Lung Cell Mol Physiol. 2006 Apr;290(4):L738-L746. (RB6-8C5, in vivo depletion, PubMed)

Alvarez MJ, Prada F, Salvatierra E, Bravo AI, Lutzky VP, Carbone C, Pitossi FJ, Chuluyan HE, Podhajcer OL. Secreted protein acidic and rich in cysteine produced by human melanoma cells modulates polymorphonuclear leukocyte recruitment and antitumor cytotoxic capacity. Cancer Res. 2005 Jun 15;65(12):5123-32. (RB6-8C5, IHC paraffin, Pubmed)

Brown CR, Blaho VA, Loiacono CM. Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol. 2003 Jul 15;171(2):893-901. (RB6-8C5, IHC paraffin, Pubmed)

Miura M, Fu X, Zhang QW, Remick DG, Fairchild RL. Neutralization of Gro alpha and macrophage inflammatory protein-2 attenuates renal ischemia/reperfusion injury. Am J Pathol. 2001 Dec;159(6):2137-45. (RB6-8C5, IHC frozen, Pubmed)

Dunussi-Joannopoulos K, Runyon K, Erickson J, Schaub RG, Hawley RG, Leonard JP. Vaccines with interleukin-12-transduced acute myeloid leukemia cells elicit very potent therapeutic and long-lasting protective immunity. Blood. 1999 Dec 15;94(12):4263-73. (RB6-8C5, IHC frozen, Pubmed)

Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399-408. (RB6-8C5, Cross-reactivity to Ly-6G and Ly-6C, Pubmed)

Hestdal K, Ruscetti FW, Ihle JN, Jacobsen SE, Dubois CM, Kopp WC, Longo DL, Keller JR. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991 Jul 1;147(1):22-8.

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