Product Information

Contents: Affinity Purified anti-mouse T- and B-cell activating antigen (GL7, Ly-77)
Catalog Number: 14-5902
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: GL-7 (GL7)
Isotype: Rat IgM

Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 μg of Purified Rat IgM Iso Cntrl (cat. 14-4341) (open histogram) or 0.25 μg of Purified GL-7 (GL7) (colored histogram) followed by PE anti-rat IgM (HIS40) (cat. 12-0990). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5902	Affinity Purified anti-mouse T- and B-cell activation antigen (GL7, Ly-77)	N/A	N/A	FC IH/F IP
13-5902	Biotin anti-mouse T- and B-cell activation antigen (GL7, Ly-77)	N/A	N/A	FC
51-5902	Alexa Fluor® 647 anti-mouse T- and B-cell activation antigen (GL7, Ly-77)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This GL7 monoclonal antibody reacts with a 35-kDa cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and a subpopulation of thymocytes which expressing high levels of CD3e. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The GL-7 (GL7) antibody has been tested by flow cytometric analysis of ConA-activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252 - 5262.

Han, S., B. Zheng, D.G. Schatz, E. Spanopoulou, and G. Kelsoe. 1996. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science 274: 2094 - 2097.

Han, S., B. Zheng, Y. Takahashi, and G. Kelsoe. 1997. Distinctive characteristics of germinal center B cells. Semin. Immunol. 9: 255 - 260.

Han, S., S.R. Dillon, B. Zheng, M. Shimoda, M.S. Schlissel, and G. Kelsoe. 1997. V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science278: 301 - 305.

Hathcock, K.S., C.E.M. Pucillo, G. Laszlo, L. Lai, and R.J. Hodes. 1995. Analysis of thymic subpopulations expressing the activation antigen GL7. Expression, genetics, and function. J. Immunol. 155: 4575 - 4581.

Related Products

Cat. 14-4341 Affinity Purified Rat IgM Isotype Control