Affinity Purified anti-mouse/human T- and B-cell activating antigen (GL7, Ly-77)

RUO: For Research Use Only

mouse GL7 antibody, Affinity Purified conjugate

Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 μg of Purified Rat IgM Iso Cntrl (cat. 14-4341) (open histogram) or 0.25 μg of Purified GL-7 (GL7) (colored histogram) followed by PE anti-rat IgM (HIS40) (cat. 12-0990). Total viable cells were used for analysis.

Contents: Affinity Purified anti-mouse/human T- and B-cell activating antigen (GL7, Ly-77)
Catalog Number: 14-5902
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Clone: GL-7 (GL7)
Host/Isotype: Rat IgM
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-5902-81	50 ug	To view relevant pricing, please select your country and resume browsing.
14-5902-82	100 ug
14-5902-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
14-4341	Affinity Purified Rat IgM Isotype Control

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
13-5902	Biotin anti-mouse/human T- and B-cell activation antigen (GL7, Ly-77)	N/A	N/A	FC	RUO
14-5902	Affinity Purified anti-mouse/human T- and B-cell activation antigen (GL7, Ly-77)	N/A	N/A	FC IH/F IP	RUO
51-5902	Alexa Fluor® 647 anti-mouse/human T- and B-cell activation antigen (GL7, Ly-77)	633	668	FC	RUO
53-5902	Alexa Fluor® 488 anti-mouse/human T- and B-cell activation antigen (GL7, Ly-77)	488	519	FC	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The GL-7 (GL7) antibody has been tested by flow cytometric analysis of ConA-activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kimura N, Ohmori K, Miyazaki K, Izawa M, Matsuzaki Y, Yasuda Y, Takematsu H, Kozutsumi Y, Moriyama A, Kannagi R. Human B-lymphocytes express alpha2-6-sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/Siglec-2. J Biol Chem. 2007 Nov 2;282(44):32200-7. (GL7, FA, human cells, PubMed)

Naito Y, Takematsu H, Koyama S, Miyake S, Yamamoto H, Fujinawa R, Sugai M, Okuno Y, Tsujimoto G, Yamaji T, Hashimoto Y, Itohara S, Kawasaki T, Suzuki A, Kozutsumi Y. Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation. Mol Cell Biol. 2007 Apr;27(8):3008-22.

Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252 - 5262.

Han, S., B. Zheng, D.G. Schatz, E. Spanopoulou, and G. Kelsoe. 1996. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science 274: 2094 - 2097.

Han, S., B. Zheng, Y. Takahashi, and G. Kelsoe. 1997. Distinctive characteristics of germinal center B cells. Semin. Immunol. 9: 255 - 260.

Han, S., S.R. Dillon, B. Zheng, M. Shimoda, M.S. Schlissel, and G. Kelsoe. 1997. V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science278: 301 - 305.

Hathcock, K.S., C.E.M. Pucillo, G. Laszlo, L. Lai, and R.J. Hodes. 1995. Analysis of thymic subpopulations expressing the activation antigen GL7. Expression, genetics, and function. J. Immunol. 155: 4575 - 4581.