Product Information

Contents: Affinity Purified anti-mouse NKG2A^B6
Catalog Number: 14-5897
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 16a11
Isotype: Mouse IgG2b, κ

Two-color surface staining of mouse splenocytes with anti-mouse NKG2AB6 (16a11) PE and anti-mouse CD3e (145-2C11) FITC. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5897	Affinity Purified anti-mouse NKG2AB6	N/A	N/A	FC
13-5897	Biotin anti-mouse NKG2AB6	N/A	N/A	FC
12-5897	PE anti-mouse NKG2AB6	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 16a11 monoclonal antibody reacts with the mouse NKG2A^B6. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD95 and are responsible for recognition of non-classical MHC class I antigen Qa-1. This antibody reacts with the C57Bl/6 mouse strain, but does not react with the BALB/c or 129 mouse strains.

When co-staining with NKG2A/C/E clone 20d5, it is important to stain for NKG2A^B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 16a11 has been reported for use in flow cytometric analysis.

Applications Tested

The 16a11 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocyte suspensions and can be used at less than or equal to 1 μg/million cells. For optimal detection of NKG2A^B6 using 16a11, a fluorochrome conjugated isotype-specific second step is recommended. If using a polyclonal anti-mouse IgG second step, please note that splenic B cells will also stain due to binding of the second step to surface IgG. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Fraser KP, Gays F, Robinson JH, van Beneden K, Leclercq G, Vance RE, Raulet DH, Brooks CG. 2002. NK cells developing in vitro from fetal mouse progenitors express at least one member of the Ly49 family that is acquired in a time-dependent and stochastic manner independently of CD94 and NKG2. Eur J Immunol. 32:868-78.
Vance RE, Jamieson AM, Cado D, Raulet DH. 2002. Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation. Proc Natl Acad Sci U S A. 99:868-73.
Kraft JR, Vance RE, Pohl J, Martin AM, Raulet DH, Jensen PE. 2000. Analysis of Qa-1(b) peptide binding specificity and the capacity of CD94/NKG2A to discriminate between Qa-1-peptide complexes. J Exp Med. 192:613-24.
Vance RE, Jamieson AM, Raulet DH. 1999. Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J Exp Med. 190:1801-12.
Vance RE, Kraft JR, Altman JD, Jensen PE, Raulet DH. 1998. Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b). J Exp Med. 188:1841-8.
Vance RE, Tanamachi DM, Hanke T, Raulet DH. 1997. Cloning of a mouse homolog of CD94 extends the family of C-type lectins on murine natural killer cells. Eur J Immunol. 27:3236-41

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