Product Information

Contents: Affinity Purified anti-mouse C1qRp (Early B lineage antigen)
Catalog Number: 14-5892
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: AA4.1
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 bone marrow cells with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.5 μg of Purified Rat IgG2b Iso Cntrl (cat. 14-4331) (left) or 0.5 μg of Purified AA4.1 (right) followed by Biotin anti-Rat IgG (cat. 13-4813) and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5892	Affinity Purified anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	N/A	N/A	FC IHC
13-5892	Biotin anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	N/A	N/A	FC
11-5892	FITC anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	488	518	FC
12-5892	PE anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	488	575	FC
17-5892	APC anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	633	660	FC
25-5892	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	488	760	FC
45-5892	PerCP-Cy5.5 anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) (PE-Cy5.5 replacement)	488	690	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1⁺ cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1⁺/CD23^-/sIgMhi), T2 (AA4.1⁺/CD23⁺/sIgMhi), and T3 (AA4.1⁺/CD23⁺/sIgMlo). AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The AA4.1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

The AA4.1 antibody has been tested by flow cytometric analysis of mouse bone marrow and splenocyte cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Dean YD, McGreal EP, Akatsu H, Gasque P. 2000 Molecular and cellular properties of the rat AA4 antigen, a C-type lectin-like receptor with structural homology to thrombomodulin. J Biol Chem. Nov 3;275(44):34382-92.
McKearn JP, Baum C, Davie JM. 1984 Cell surface antigens expressed by subsets of pre-B cells and B cells. J Immunol. Jan;132(1):332-9.

Related Products

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Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)