Product Information

Contents: Affinity Purified anti-mouse Ly-49G
Catalog Number: 14-5885
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: AT-8 (AT8)
Isotype: Mouse IgG2a, κ

Staining of BALB/c splenocytes with APC anti-mouse pan-NK (DX5) and PE mouse IgG2a isotype control (left) or PE AT-8 (AT8) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5885	Affinity Purified anti-mouse Ly-49G (Ly49G)	N/A	N/A	FC IP
13-5885	Biotin anti-mouse Ly-49G (Ly49G)	N/A	N/A	FC
12-5885	Phycoerythrin (PE) anti-mouse Ly-49G (Ly49G)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The AT8 monoclonal antibody reacts with the mouse Ly-49G receptor from 129, FVB, BALB/c, C3H, CBA, SJL and A/J, but not C57Bl/6 mice. Ly49G, a member of the Ly49 family is expressed by NK cell subset.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The AT-8 (AT8) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

The AT-8 (AT8) antibody has been tested by flow cytometric analysis of BALB/c mouse splenocyte suspension. This can be used at less than or equal to 2.0 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Makrigiannis AP, Rousselle E, Anderson SK. 2004. Independent control of Ly49g alleles: implications for NK cell repertoire selection and tumor cell killing. J Immunol. 172(3):1414-25.

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