mouse Dendritic Cell Marker (33D1) antibody: purified antibody

Contents: Affinity Purified anti-mouse Dendritic Cell Marker (33D1)
Catalog Number: 14-5884
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 33D1
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-5884-81	50 ug	$60
14-5884-82	100 ug	$90
14-5884-85	500 ug	$220

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with 0.25 µg of purified rat IgG2b isotype control (cat.14-4331) (open histogram) or 0.25 µg of purified 33D1 (colored histogram) followed by biotin anti-rat IgG (cat.13-4813) and SAv-PE (cat.12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5884	Affinity Purified anti-mouse Dendritic Cell (DC) Marker (33D1)	N/A	N/A	FA FC IH/F
13-5884	Biotin anti-mouse Dendritic Cell (DC) Marker (33D1)	N/A	N/A	FC
12-5884	PE anti-mouse Dendritic Cell (DC) Marker (33D1)	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 33D1 monoclonal antibody reacts with a mouse DC-specific surface marker. The nature and biological activity of the 33D1 antigen is as of yet unknown. 33D1 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer’s patch. Bone marrow dendritic cells require GM-CSF to express the 33D1 antigen and this expression is down regulated in the presence of IL-4. 33D1 antigen has been detected in vivo in brain dendritic cells post infection via Toxoplasma gondii.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining. It has also been reported in complement-mediated cytotoxicity. (Please use Functional Grade purified 33D1 in functional assays.)

Applications Tested

The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Special Notes

For detection of purified 33D1 it is recommended not to use goat anti-rat due to it's low affinity for 33D1. Rather, we recommend using biotin mouse anti-rat (cat. 13-4813).

References

Nussenzweig, M.C., R.M. Steinman, M.D. Witmer, and B. Gutchinov. 1982. A monoclonal antibody specific for mouse dendritic cells. Proc. Natl. Acad. Sci. USA 79: 161 - 165.

Steinman, R.M., B. Gutchinov, M.D. Witmer, and M.C. Nussenzweig. 1983. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157: 613 - 627.

Inaba, K., M. Inaba, N. Romani, H. Aya, M. Deguchi, S. Ikehara, S. Muramatsu, and R.M. Steinman. 1992. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176: 1693 - 1702.

Masurier, C., C. Pioche-Durieu, B.M. Colombo, R. Lacave, F.M. Lemoine, D. Klatzmann, and M. Guigon. 1999. Immunophenotypical and functional heterogeneity of dendritic cells generated from murine bone marrow cultured with different cytokine combinations: implications for anti-tumoral cell therapy. Immunology 96: 569 - 577.

Fischer, H.-G., U. Bonifas, and G. Reichmann. 2000. Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with Toxoplasma gondii. J. Immunol. 164: 4826 - 4834.

Dudziak D, Kamphorst AO, et al. 2007. Differential antigen processing by dendritic cell subsets in vivo. Science 315(5808):107-11. (33D1, IHC frozen, PubMed)

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