Product Information

Contents: Affinity Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2)
Catalog Number: 14-5883
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MD5-1
Isotype: Armenian Hamster IgG

Staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.125 µg FG Armenian hamster IgG isotype control (cat.16-4444) (open histogram) or 0.125 µg purified anti-mouse DR5 (MD5-1) (colored histogram) followed by biotin anti-Armenian hamster IgG (cat.13-4113) and SAv-PE (cat.12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5883	Affinity Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FA FC
16-5883	Functional Grade* Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FA FC
13-5883	Biotin anti-mouse DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FC
12-5883	PE anti-mouse DR5 (CD262, Trail-R2, TrailR2)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MD5-1 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays. (Please use Functional Grade purified MD5-1, cat. 16-5883, in functional assays.)

Applications Tested

The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sheridan, J. P., S. A. Marster, R. M. Pitti, A. Gurney, M. Skubatch, D. Baldwin, L. Ramakrishnan, C. L. Gray, K. Baker, W. I. Wood, A. D. Goddard, P. Godowski, A. Ashkenazi. 1997. Control of TRAIL-Induced Apoptosis by a Family of Signaling and Decoy Receptors. Science 277:818-821.
Takeda K, Yamaguchi N, Akiba H, Kojima Y, Hayakawa Y, Tanner JE, Sayers TJ, Seki N, Okumura K, Yagita H, Smyth MJ. 2004. Induction of Tumor-specific T Cell Immunity by Anti-DR5 Antibody Therapy. J Exp Med. Feb 16;199(4):437-48. Epub 2004 Feb 09.

Related Products

Cat. 11-4111  FITC Anti-Armenian Hamster IgG
Cat. 13-4113  Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4444  Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 14-4888  Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)