Product Information

Contents: Affinity Purified anti-mouse RAE-1g (RAE-1-gamma, RAE-1-g, RAE1-g)
Catalog Number: 14-5881
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: CX1
Isotype: Rat IgG2b, κ

Staining of Yac-1 cells with 0.125 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or 0.125 μg of Purified CX1 (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5881	Affinity Purified anti-mouse RAE-1g (RAE-1-gamma, RAE-1-g, RAE1-g)	N/A	N/A	FA FC
16-5881	Functional Grade* Purified anti-mouse RAE-1g (RAE-1-gamma, RAE-1-g, RAE1-g)	N/A	N/A	FA FC
13-5881	Biotin anti-mouse RAE-1g (RAE-1-gamma, RAE-1-g, RAE1-g)	N/A	N/A	FC
12-5881	Phycoerythrin (PE) anti-mouse RAE-1g (RAE-1-gamma, RAE-1-g, RAE1-g)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CX1 monoclonal antibody reacts with the mouse retinoic acid early inducible gamma (RAE-1γ) and weakly cross-reacts with the RAE-1α and β, but not with δ and ε. These 5 members of the RAE-1 family, along with another protein encoded by the minor histocompatibility gene complex called H60 have been identified as ligands for the mouse NKG2D molecule. These ligands are normally absent from adult tissue, however the presence of retinoic acid or certain disease conditions (such as tumors) can induce up-regulation of this protein at the cell surface. RAE-1α, -β, and -γ and H60 are expressed in BALB/c mice, but not in C57BL/6, whereas RAE-1δ, and -ε are expressed in C57BL/6 mice. It is reported that in NOD mice, both NKG2D and its ligand RAE-1 are expressed on the same NK cells, resulting in self-modulation of NKG2D expression and function.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CX1 antibody has been reported for use in flow cytometric analysis. it has also been reported in blocking of NKG2D to RAE-1 in functional assays. (Please use Functional Grade purified CX1, cat. 16-5881, in functional assays.)

Applications Tested

The CX1 antibody has been tested by flow cytometric analysis of YAC-1 cell line and mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Cerwenka, A., A.B. Bakker, T. McClanahan, J. Wagner, J. Wu, J.H. Phillips, L.L. Lanier. (2000). “Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice.” Immunity. 12(6): 721-27.
Diefenbach, A., E.R. Jensen, A.M. Jamieson, D.H. Raulet. (2001). “Rae-1 and H60 ligands of the NKG2D receptor stimulate tumour immunity.” Nature. 413(6852): 165-71.
Lodoen, M., K. Ogasawara, J.A. Hamerman, H. Arase, J.P. Houchins, E.S. Mocarski, L.L. Lanier. (2003). “NKG2D-mediated natural killer cell protection against cytomegalovirus is impaired by viral gp40 modulation of retinoic acid early inducible 1 gene molecules.” 197(10): 1245-53.

Related Products

Cat. 14-4031  Purified Rat IgG2b Isotype Control
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)